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PhD Public Seminar: Yimei Jin, MS, MD

When & Where

April 17
1:00 PM - 2:00 PM
3SCR, 1881 East Road, 77054, 3SCR3.3202ab (View in Google Map)

Contact

Event Description

Tsyn-seq: A T cell Synapse-based Antigen Identification Platform

Advisor: Robert R. Jenq, MD

Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets of T-cell receptors (TCRs) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APCs) with a Fas-inducible NF-κB reporter and T cells with a nuclear factor of activated T cells (NFAT) reporter. To functionally screen for target antigens from a cDNA library, productively interacting T cell–APC aggregates were detected by dual reporter activity and enriched by flow sorting followed by antigen identification quantified by deep sequencing (Tsyn-seq). When applied to a previously characterized TCR specific for the E7 antigen derived from human papillomavirus type 16 (HPV16), Tsyn-seq successfully enriched the correct cognate antigen from a cDNA library derived from an HPV16-positive cervical cancer cell line. Tsyn-seq provides a method for rapidly identifying antigens recognized by TCRs of interest from a tumor cDNA library.

Advisory Committee:
Robert R. Jenq, MD, Chair
Matthew M. Gubin, PhD
Stephanie S. Watowich, PhD
Gregory A. Lizee, PhD
Linghua Wang, MD, PhD
Nadim J. Ajami, PhD

Attend via Zoom
Password:  188327

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Tsyn-seq: A T cell Synapse-based Antigen Identification Platform

Advisor: Robert R. Jenq, MD

Tools for genome-wide rapid identification of peptide–major histocompatibility complex targets of T-cell receptors (TCRs) are not yet universally available. We present a new antigen screening method, the T-synapse (Tsyn) reporter system, which includes antigen-presenting cells (APCs) with a Fas-inducible NF-κB reporter and T cells with a nuclear factor of activated T cells (NFAT) reporter. To functionally screen for target antigens from a cDNA library, productively interacting T cell–APC aggregates were detected by dual reporter activity and enriched by flow sorting followed by antigen identification quantified by deep sequencing (Tsyn-seq). When applied to a previously characterized TCR specific for the E7 antigen derived from human papillomavirus type 16 (HPV16), Tsyn-seq successfully enriched the correct cognate antigen from a cDNA library derived from an HPV16-positive cervical cancer cell line. Tsyn-seq provides a method for rapidly identifying antigens recognized by TCRs of interest from a tumor cDNA library.

Advisory Committee:
Robert R. Jenq, MD, Chair
Matthew M. Gubin, PhD
Stephanie S. Watowich, PhD
Gregory A. Lizee, PhD
Linghua Wang, MD, PhD
Nadim J. Ajami, PhD

Attend via Zoom
Password:  188327

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